Publications

2025

Rai M, Okar P, Shefali SA, Fitt AJ, Shen MZ, Molomjamts M, Pepin R, Nemkov T, D’Alessandro A, Tennessen JM. (2025). New alleles of D-2-hydroxyglutarate dehydrogenase enable studies of oncometabolite function in Drosophila melanogaster. G3 Genes|Genomes|Genetics. jkaf132. doi: 10.1093/g3journal/jkaf132

Rai M, Li H, Policastro RA, Pepin R, Zentner GE, Nemkov T, D’Alessandro A, Tennessen JM. (2025). Glycolytic disruption restricts Drosophila melanogaster larval growth via the cytokine Upd3. PLOS Genetics. 21(5): e1011690. doi: 10.1371/journal.pgen.1011690

Fasteen TD, Hernandez MR, Policastro RA, Sterrett MC, Zentner GE, Tennessen JM. (2025). Journal of Lipid Research. 66(6): 100815. doi: 10.1016/j.jlr.2025.100815

Heidarian Y, Fasteen TD, Mungcal L, Buddika K, Mahmoudzadeh NH, Nemkov T, D’Alessandro A, Tennessen JM. (2025). Hypoxia-inducible factor 1α is required to establish the larval glycolytic program in Drosophila melanogaster. Molecular Metabolism. 93: 102106. doi: 10.1016/j.molmet.2025.102106

2024

Mesmar F, Muhsen M, Mirchandani R, Tourigny JP, Tennessen JM, Bondesson M. (2024). The herbicide acetochlor causes lipid peroxidation by inhibition of glutathione peroxidase activity. Toxicological Sciences. 202(2): 302-313. doi: 10.1093/toxsci/kfae113

Mahmoudzadeh NH, Heidarian Y, Tourigny JP, Fitt AJ, Beebe K, Li H, Luhur A, Buddika K, Mungcal L, Kundu A, Policastro RA, Brinkley GJ, Zentner GE, Nemkov T, Pepin R, Chawla G, Sudarshan S, Rodan AR, D’Alessandro A, Tennessen JM. (2024). Renal L-2-hydroxyglutarate dehydrogenase activity promotes hypoxia tolerance and mitochondrial metabolism in Drosophila melanogaster. Molecular Metabolism. 89: 102013. doi: 10.1016/j.molmet.2024.102013

Kundu A, Brinkley GJ, Nam H, Karki S, Kirkman R, Pandit M, Shim E, Widden H, Lio J, Heidarian Y, Mahmoudzadeh NH, Fitt AJ, Absher D, Ding H, Crossman DK, Placzek WJ, Locasale JW, Rakheja D, McConathy JE, Ramachandran R, Bae S, Tennessen JM, Sudarshan S. (2024). L-2-hydroxyglutarate remodeling of the epigenome and epitranscriptome creates metabolic vulnerability in kidney cancer models. J Clin Invest. 134(13):e171294. doi: 10.1172/JCI171294

Garfinkel AM, Ilker E, Mitazawa H, Schmeisser K, Tennessen JM. (2024). Historic obstacles and emerging opportunities in the field of developmental metabolism — lessens from Heidelberg. Development. 151(12): dev202937. doi: 10.1242/dev.202937

Hayot G, Masser R, Lloyd G, Keith N, Diwan G, Martinez Lopez R, Barnard M, von Clausbruch CA, Grasse N, Smoot S, Escher B, Tennessen JM, Tindall A, Oliver B, Shaw J, Scholz S, Freedman J, Straehle U, Colburne J, Weiss C, Dickmeis T. (2024). Systematic acquisition of toxicity data in non-sentient models across animal phylogeny: implications for read-across and estimation of toxicity in humans. Naunyn-Schmiedeberg’s archives of pharmacology. 397(1): 17-69. doi: 10.1007/s00210-024-02974-3

Yang S, Niou ZX, Enriquez A, LaMar J, Huang JY, Ling K, Jafar-Nejad P, Gilley J, Coleman MP, Tennessen JM, Rangaraju V, Lu HC. (2024). NMNAT2 supports vesicular glycolysis via NAD homeostasis to fuel fast axonal transport. Mol Neurodegeneration. 19: 13. doi: 10.1186/s13024-023-00690-9

Fleck SA, Biswas P, DeWitt ED, Knuteson RL, Eisman RC, Nemkov T, D’Alessandro A, Tennessen JM, Rideout E, Weaver LN. (2024). Auxin exposure disrupts feeding behavior and fatty acid metabolism in adult Drosophila. eLife. 12:RP91953. doi: 10.7554/eLife.91953.3

Rai M, Carter SM, Shefali SA, Chawla G, Tennessen JM. (2024). Characterization of genetic and molecular tools for studying the endogenous expression of Lactate dehydrogenase melanogaster. PLOS ONE. 19(1): e0287865. doi: 10.1371/journal.pone.0287865

Heidarian Y, Tourigny JP, Fasteen TD, Mahmoudzadeh NH, Hurlburt AJ, Nemkov T, Reisz JA, D’Alessandro A, Tennessen JM. (2024). Metabolomic analysis of Drosophila melanogaster larvae lacking pyruvate kinase. G3 Genes|Genomes|Genetics. 14(1): jkad228. doi: 10.1093/g3journal.jkad228

2023

Rand MD, Tennessen JM, Mackay TFC, Anholt RRH. (2023). Perspectives of the Drosophila melanogaster model for advances in toxicological science. Current Protocols. 3, e870. doi: 10.1002/cpz1.870

Holsopple JM, Smoot SR, Popodi EM, Colburne JK, Shaw JR, Oliver B, Kaufman TC, Tennessen JM. (2023). Assessment of chemical toxicity in adult Drosophila melanogaster. J Vis Exp. (193):10.3791/62029 . doi: 10.3791/65029

Frame AK, Robinson JW, Mahmoudzadeh NH, Tennessen JM, Simon AF, Cumming RC. Aging and memory are altered by genetically manipulating lactate dehydrogenase in the neurons or glia of flies. Aging (Albany NY). 15(4): 947-981. doi: 10.18632/aging.204565

2022

Rai M, Carter SM§, Shefali SA, Mahmoudzadeh NH, Pepin R, Tennessen JM. (2022). The Drosophila melanogasterenzyme glycerol-3-phosphate dehydrogenase 1 is required for oogenesis, embryonic development, and amino acid homeostasis. G3 Genes|Genomes|Genetics. 12(8): jkac115. doi: 10.1093/g3journal/jkac115

Taub M, Mahmoudzadeh NH, Tennessen JM, Sudarshan S. (2022). Renal oncometabolite L-2-hydroxyglutarate imposes a block in kidney tubulogenesis: evidence for an epigenetic basis for the L-2HG induced impairment of differentiation. Front Endocrinol. 13: 2022. doi: 10.3389/fendo.2022.932286

Parish AJ, Rice DW, Tanquary VM, Tennessen JM, Newton ILG. (2022). Honey bee symbiont buffers larvae against nutritional stress and supplements lysine. The ISME Journal. 16(9): 2160-2168. doi: 10.1038/s41396-022-01268-x

2021

Tennessen JM. (2021). Ecdysone and 20-hydroxyecdysone are not required to activate glycolytic gene expression in Drosophila melanogaster embryos. Micropublications Biology. 2021:10.17912/micropub.biology.000501. doi: 10.17912/micropub.biology.000501

Gillette CM, Tennessen JM, Reis T. (2021) Balancing energy expenditure and storage with growth and biosynthesis during Drosophila development. Developmental Biology. 475: 234-244. doi: 10.1016/j.ydbio.2021.01.019

Pandey M, Bansal S, Bar S, Sokol NS, Tennessen JM, Kapahi P, Chawla G. (2021). miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in Drosophila. eLife. 10:e62621. doi: 10.7554/eLife.62621

2020

Mahmoudzadeh N, Fitt A§, Schwab DB, Brinkley GJ, Martenis N§, Neese L, Li H, Owings CG, Karty JA, Hardy RW, Moczek AP, Sudarshan S, Picard CJ, and Tennessen JM. (2020). Dipteran larvae accumulate high levels of the oncometabolite L-2-hydroxyglutarate. Insect Biochemistry and Molecular Biology. 127:103493. doi: 10.1016/j.ibmb.2020.103493

Luhur A, Mariappa D, Klueg KM, Buddika K, Tennessen JM, Zelhof AC. (2020). Adapting Drosophila melanogastercell lines to serum-free culture conditions. G3:Genes|Genomes|Genetics. 10(12):4541-4551. doi: 10.1534/g3.120.401769

Brinkley G, Nam H, Shim E, Kirkman R, Kundu A, Karki S, Heidarian Y, Tennessen JM, Liu J, Locasale J, Guo T, Wei S, Gordetsky J, Johnson-Pais T, Absher D, Rakheja D, Kumar Challa A, and Sudarshan S. (2020). Teleological Role of L-2-Hydroxyglutarate Dehydrogenase in the Kidney. Disease Models and Mechanisms. 13(11): dmm045898. doi: 10.1242/dmm.045898

Bawa S, Brooks DS, Neville KE, Tipping M, Sagar MA, Kollhoff JA, Chawla C, Geisbrecht BV, Tennessen JM, Eliceiri KW, and Geisbrecht ER. (2020). Drosophila TRIM32 cooperates with glycolytic enzymes to promote cell growth. eLife. 9:e52358. doi: 10.7554/eLife.52358

Drummond-Barbosa D, Tennessen JM. (2020) Reclaiming warburg: using developmental biology to gain insight into human metabolic diseases. Development. 147(11): dev189340. doi: 10.1242/dev.189340

2019

Li H, Rai M, Buddika K, Sterrett MC, Luhur A, Mahmoudzadeh NH, Julick CR, Pletcher RC, Chawla G, Gosney CJ, Burton AK, Karty JA, Montooth KL, Sokol NS, Tennessen JM. Lactate dehydrogenase and glycerol-3-phosphate dehydrogenase cooperatively regulate growth and carbohydrate metabolism during Drosophila melanogaster larval development. Development. pii: dev.175315. doi: 10.1242/dev.175315.

Li H, Tennessen JM. (2019). Quantification of D- and L-2-Hydroxyglutarate in Drosophila melanogaster Tissue Samples Using Gas Chromatography-Mass Spectrometry. Methods Mol Biol. 1978: 155–165. doi: 10.1007/978-1-4939-9236-2_10

Pletcher RC, Hardman SL, Intagliata SF, Lawson RL, Page A, Tennessen JM. (2019). A Genetic Screen Using the Drosophila melanogaster TRiP RNAi Collection To Identify Metabolic Enzymes Required for Eye Development. G3: Genes | Genomes | Genetics. 9(7):2061-2070. doi: 10.1534/g3.119.400193

Krejčová G, Danielová A, Nedbalová P, Kazek M, Strych L, Chawla G, Tennessen JM, Lieskovská J, Jindra M, Doležal T, and Bajgar A. (2019). Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense. eLife. 8:e50414. doi: 10.7554/eLife.50414

2018

Maiuri AR, Li H, Stein BD, Tennessen JM, O’Hagan HM. (2018). Inflammation-induced DNA methylation of DNA polymerase gamma alters the metabolic profile of colon tumors. Cancer Metab. 6(9): 1-11. doi: 10.1186/s40170-018-0182-7

Li H and Tennessen JM. (2018). Preparation of Drosophila larval samples for gas chromatography-mass spectrometry (GC-MS)-based metabolomics. Journal of Visualized Experiments. 136: e57847. doi: 10.3791/57847

Li H, Hurlburt AJ, and Tennessen JM. (2018). A Drosophila model of combined D-2- and L-2-hydroxyglutaric aciduria reveals a mechanism linking mitochondrial citrate export with oncometabolite accumulation.  Disease Models and Mechanisms. 11(9): dmm035337. doi: 10.1242/dmm.035337

Osburn K, Amaral J, Metcalf SR, Nickens DM, Rogers CM, Sausen C, Caputo R, Miller J, Li H, Tennessen JM, Bochman, M. L. (2018). Primary souring : A novel bacteria-free method for sour beer production. Food Microbiology, 70, 76–84. doi: 10.1016/j.fm.2017.09.007

2017

St. Clair SL, Li H, Ashraf U, Karty JA, Tennessen JM. (2017) Metabolic analysis reveals that the Drosophila melanogaster gene lysine influences diverse aspects of metabolism. Genetics. 207(4): 1255-1261. doi: 10.1534/genetics.117.300201

Krejci A, Tennessen JM. (2017) Metabolism in time and space — exploring the frontier of developmental biology. Development. 144(18): 3193-3198. doi: 10.1242/dev.150573

Li H, Tennessen JM. (2017). Methods for studying the metabolic basis of Drosophila development. WIREs Dev Biol. 6: e280. doi: 10.1002/wdev.280

Zhang W, Orgando DG, Kim ET, Choi MJ, Li H, Tennessen JM, Bonanno JA. (2017) Conditionally immortal Slc4a11-/- mouse corneal endothelial cell line recapitulates disrupted glutaminolysis seen in Slc4a11-/- mouse model. Invest Ophthalmol. 58(9): 3723-3731. doi: 10.1167/iovs.17-21781

Cox JE, Thummel CS, Tennessen JM. (2017). Metabolomic Studies in Drosophila. Genetics. 206(3): 1169–1185.

Li H, Chawla G, Hurlburt AJ, Sterrett MC, Zaslaver O, Cox J, Karty JA, Rosebrock AP, Caudy AA, Tennessen JM. (2017). Drosophila larvae synthesize the putative oncometabolite L-2-hydroxyglutarate during normal developmental growth, 114(6), 2–7. doi: 10.1073/pnas.1614102114

Zhang W, Li H, Ogando DG, Shimin L, Feng M, Price FW, Tennessen JM, Bonanno JA. Glutaminolysis is essential for energy production and ion transport in human corneal endothelium. EBioMedicine. 16: 292-301. doi: 10.1016/j.ebiom.2017.01.004

Previous (selected)

Tennessen JM, Barry WE, Cox J, Thummel CS (2014). Methods for studying metabolism in Drosophila. Methods, 68, 105–115. doi: 10.1016/j.ymeth.2014.02.034

Tennessen JM, Bertagnolli NM, Evans J, Sieber MH, Cox J, Thummel CS. (2014). Coordinated Metabolic Transitions During Drosophila Embryogenesis and the Onset of Aerobic Glycolysis, 4(May), 839–850. doi: 10.1534/g3.114.010652

Tennessen JM, Thummel CS. (2011). Coordinating growth and maturation - Insights from Drosophila. Current Biology, 21(18), R750–R757. doi:10.1016/j.cub.2011.06.033

Tennessen JM, Baker KD, Lam G, Evans J, Thummel CS. (2011). Article The Drosophila Estrogen-Related Receptor Directs a Metabolic Switch that Supports Developmental Growth. Cell Metabolism, 13(2), 139–148. doi: 10.1016/j.cmet.2011.01.005

Palanker L, Tennessen JM, Lam G, Thummel CS. (2009). Article Drosophila HNF4 Regulates Lipid Mobilization and b -Oxidation. Cell Metabolism, 9(3), 228–239. doi: 10.1016/j.cmet.2009.01.009